GREEN EXTRACTION OF BIOACTIVE COMPOUNDS FROM FRUITS AND VEGETABLES WASTE AS A PRECURSOR OF NUTRACEUTICALS
DOI:
https://doi.org/10.58475/2025.63.3.2208Keywords:
Brassica oleracea, pisum sativum, food waste, polyphenols, antioxidant capacity, nutraceutical, sustainability, PakistanAbstract
The global population is experiencing rapid growth, resulting in significant challenges related to disease prevalence, food security, healthcare systems, and nutrition. The processing of fruits and vegetables generates vast quantities of agro-waste. The disposal of agro-waste represents both a cost to the processor and poses potential negative impacts on the environment. The valorization of these waste materials into the extraction of bioactive compounds with nutraceutical potential serves as a new pathway for sustainable utilization. The present study was aimed to prepare the aqueous extracts of cabbage (Brassica oleracea L. var. capitata) waste, grapefruit (Citrus paradisi Macfad) peels and pea (Pisum sativum) pods using different methods such as extraction at room temperature and boiling for determination of various bioactive compounds. Quantitative analysis revealed that cabbage extracts contained the highest phenolic contents (161.62 mg GAE/g) among the other extracts. Grapefruit peels demonstrated the highest tannin contents (33.56 mg tannins/g), while pea pod extracts were notable for flavonoid contents (31.83 mg QE/g). Cabbage extracts also exhibited substantial reducing sugar (136.96 mg glucose/g) and total sugar (560.2 mg glucose/g). However, there were no significant variations (p > 0.05) among the bioactive compounds in cabbage waste, grapefruit peels, and pea pods , when compared with each other. So, aqueous green extracts of these plant material could be used as saver nutritional agent as compared to the other conventional non-greener extracts. These could be a stimulator for sustainable and economical nutraceutical future.
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Abbas, M., F. Saeed, F.M. Anjum, M. Afzaal, T. Tufail, M.S. Bashir, A. Ishtiaq, S. Hussain and H.A.R. Suleria. 2017. Natural polyphenols: an overview. Int. J. Food Prop. 20:1689-1699.
Akter, B., M.R. Salleh, M.H. Abu Bakar, T.J. Shun and C.L. Hoong. 2022. Utilisation of watermelon, pineapple and banana fruit peels as prebiotics and their effect on growth of probiotic. Int. J. Food Sci. Technol. 57:7359-7367.
Aqilah, N., K. Rovina, W. Felicia and J. Vonnie. 2023. A review on the potential bioactive components in fruits and vegetable wastes as value-added products in the food industry. Molecules. 28:2631.
Basri, M., A. Karim, A. Sulaiman, M.A. Tawakkal, M. Nor, S. Ariffin, A. Ghani and M. Salleh. 2021. Progress in the valorization of fruit and vegetable wastes: active packaging, biocomposites, by-products, and innovative technologies used for bioactive compound extraction. Polymers. 13:3503.
Bhandari, S.R., C.S. Choi, J. Rhee, J.S. Jo, Y.K. Shin, J.W. Song and J.G. Lee. 2021. Seasonal variation in agronomic characteristics and sugar content of cabbage genotypes. Chil. J. Agric. Res. 81(1):80-9
Castaldo, L., L. Izzo, A. Gaspari, S. Lombardi, Y. Rodríguez-Carrasco, A. Narváez, M. Grosso and A. Ritieni. 2021. Chemical composition of green pea (Pisum sativum L.) pod extracts and their potential exploitation as ingredients in nutraceutical formulations. Antioxidants. 11:105.
Chung, K.T., T.Y. Wong, C.I. Wei, Y.W. Huang and Y. Lin. 1998. Tannins and human health: a review. Crit. Rev. Food Sci. Nutr. 38:421-464.
Czech, A., A. Malik, B. Sosnowska and P. Domaradzki. 2021. Bioactive substances, heavy metals, and antioxidant activity in whole fruit, peel, and pulp of citrus fruits. Int. J. Food Sci. 2021:1-14.
Devi, P., G. Singh, Y.S. Rattanpal, M.H. Gupta and R. Lhamu. 2024. Potential value of bioactive and enzymatic antioxidant compounds in grapefruit (citrus × paradisi Macf.) varieties. Food Anal. Methods. 17:834-846.
Dubale, S., D. Kebebe, A. Zeynudin, N. Abdissa and S. Suleman. 2023. Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. J. Exp. Pharmacol. 15:51-62.
Elshaer, S.E., G.M. Hamad, E.E. Hafez, H.H. Baghdadi, F.M. El-Demerdash and J. Simal Gandara. 2022. Root extracts of Saussurea costus as prospective detoxifying food additive against sodium nitrite toxicity in male rats. Food Chem. Toxicol. 166:113225.
Erken, O. and S. Kaya, 2017. Free radical scavenging activity, phenolic. Fresenius Environ. Bull. 26: 4383-4389.
Ezeabara, C.A., C.F. Ikeh, C.V. Ilodibia, B.O. Aziagba, O.E. Okanume and E.I. Mbaekwe. 2014. Comparative determination of 214 phytochemical, proximate and mineral compositions in various parts of Portulaca oleracea L. J. Plant Sci. 2:293-298.
Fendri, L.B., F. Chaari, F. Kallel, M. Koubaa, S. Zouari-Ellouzi, I. Kacem, S.E. Chaabouni and D. Ghribi-Aydi. 2022. Antioxidant and antimicrobial activities of polyphenols extracted from pea and broad bean pods wastes. J. Food Meas. Charact. 16:4822-4832
Gaur, S.R., J. Nishad, N. Jakhar and C. Kaur. 2015. Food industry waste: mine of nutraceuticals. Int. J. Sci. Environ. Technol. 4:205-229.
Ghosh, D. 2015. Tannins from foods to combat diseases. Int J Pharm Res Rev. 4:40-44.
Gomiero, T. 2016. Soil degradation, land scarcity and food security: reviewing a complex challenge. Sustainability. 8:281
Goran, M. 2023. Impacts of population growth and economic development on food security in low-income and middle-income countries. Front. Hum. Dyn. 5:2673-2726.
Gowe, C. 2015. Review on potential use of fruit and vegetables by-products as a valuable source of natural food additives. Food Sci. Qual. Manag. 45:47-61
Ignat, I., I. Volf and V.I. Popa. 2011. A critical review of methods for characterization of polyphenolic compounds in fruits and vegetables. Food chemistry. 126:1821-1835.
Kaur, C. and H.C. Kapoor. 2002. Anti‐oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Technol. 37:153-161.
Khan, J., P.K. Deb, S. Priya, K.D. Medina, R. Devi, S.G. Walode and M. Rudrapal. 2021. Dietary flavonoids: Cardioprotective potential with antioxidant effects and their pharmacokinetic, toxicological and therapeutic concerns. Molecules. 26:4021.
Langyan, S., P. Yadava, F.N. Khan, Z.A. Dar, R. Singh and A. Kumar. 2022. Sustaining protein nutrition through plant based foods. Front. Nutr. 8:772573.
Lima, G.P.P., F. Vianello, C.R. Corrêa, R.A.D.S. Campos and M.G. Borguini. 2014. Polyphenols in fruits and vegetables and its effect on human health. Food Nutr. Sci. 5:1065-1082
Mateos-Aparicio, I., A. Redondo-Cuenca, M.J. Villanueva-Suárez, M.A. Zapata-Revilla and M.D. Tenorio-Sanz. 2010. Pea pod, broad bean pod and okara, potential sources of functional compounds. LWT Food Sci. Technol. 43:1467-1470.
Munir, H., S. Yaqoob, A. Awan, K. Imtiaz, A. Naveed, N. Ahmad and Y. Ma. 2024. Unveiling the chemistry of citrus peel: insights into nutraceutical potential and therapeutic applications. Foods. 13:1681.
Rambabu, K., J. AlYammahi, A. Thanigaivelan, G. Bharath, N. Sivarajasekar, S. Velu and F. Banat. 2022. Sub-critical water extraction of reducing sugars and phenolic compounds from date palm fruit. Biomass Convers. Biorefin. 1-12. Ravindran, R. and R. Jaiswal. 2016. Exploitation of Food Industry Waste for High Value Products. Trends Biotechnol. 34:58-69.
Podsędek, A., D. Sosnowska, M. Redzynia and B. Anders. 2006. Antioxidant capacity and content of Brassica oleracea dietary antioxidants. Int. J. Food Sci. Technol. 41:49-58.
Samtiya, M., R.E. Aluko, T. Dhewa and J.M. Moreno Rojas. 2021. Potential Health Benefits of Plant Food-Derived Bioactive Components: An Overview. Foods (Basel, Switzerland). 10:839.
Shirazi, O. U., M.M.A.K. Khattak, N.A.M. Shukri and M.N. Nasyriq. 2014. Determination of total phenolic, flavonoid content and free radical scavenging activities of common herbs and spices. J. Pharmacogn. Phytochem. 3:104-108.
Suleria, H.A., C.J. Barrow and F.R. Dunshea. 2020. Screening and characterization of phenolic compounds and their antioxidant capacity in different fruit peels. Foods. 9(9):1206.
Sir Elkhatim, K. A., R.A. Elagib and A.B. Hassan. 2018. Content of phenolic compounds and vitamin C and antioxidant activity in wasted parts of Sudanese citrus fruits. Food Sci. Nutr. 6:1214-1219.
Singh, V., T.S. Chahal, S.K. Grewal and P.S. Gill. 2021. Effect of fruit development stages on antioxidant properties and bioactive compounds in peel, pulp and juice of grapefruit varieties. J. Food Meas. Charact. 15(3):2531-2539.
Tamilselvi, N., P. Krishnamoorthy, R. Dhamotharan, P. Arumugam and E. Sagadevan. 2012. Analysis of total phenols, total tannins and screening of phytocomponents in Indigofera aspalathoides (Shivanar Vembu) Vahl EX DC. J. Chem. Pharm. Res. 4:3259- 3262.
Terao, J. 2017. Factors modulating bioavailability of quercetin-related flavonoids and the consequences of their vascular function. Biochem. Pharmacol. 139:15-23.
Townsend, R. F., S. Jaffee, Y.T. Hoberg and A. Htenas. 2016. Future of Food: Shaping the Global Food System to Deliver Improved Nutrition and Health. 32.
Ullah, A., S. Munir, S.L. Badshah, N. Khan, L. Ghani, B.G. Poulson, A.H. Emwas and M. Jaremko. 2020. Important Flavonoids and Their Role as a Therapeutic Agent. Molecules. 25:5243.
UNICEF. 2023. Malnutrition in Children - UNICEF Report. WHO, 2023. ”WHO Regional Office for Europe nutrient poofile model” In WHO regional office for Europe nutrient profile model. Zaki, N. and M. Naeem. 2021. Antioxidant, antimicrobial and anticancer activities of citrus peels to improve the shelf life of yoghurt drink. Egypt. J. Food Sci. 49:249-265.
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